New in ACS Synthetic Biology: Optimization of a High-Throughput Screen for Monitoring Disease-Linked Protein Misfolding in Bacteria
Skretas Lab has been leveraging the fluorescence of Escherichia coli bacteria engineered to express fusions of misfolding-prone (MisP) human proteins with green fluorescent protein (GFP) as a high-throughput screening readout for identifying chemical compounds that rescue disease-associated protein misfolding and aggregation. In a newly published study in ACS Synthetic Biology, the team defined conditions that…
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